FIGURE 12.

CD157 binding to FN induces FAK and c-SRC activation. A, Met-5A/scramble and Met-5A/shCD157 were starved overnight and plated for 1 h at 37 °C onto FN-coated plates. Adherent cells were lysed, and cleared lysates were subjected to Western blot. Membranes were first probed with anti-phospho-FAK, phospho-c-SRC, or phospho-Akt, stripped, and re-probed with anti-FAK, -c-SRC, or -Akt, followed by incubation with the appropriate HRP-conjugated antibodies. B, phosphorylation of FAK, c-SRC, and Akt in Met-5A/shCD157 compared with Met-5A/scramble was quantified using the software package ImageJ 1.43 (National Institutes of Health). Results are the means ± S.E. of three experiments and are expressed as fold decrease in phosphorylation in Met-5A/shCD157 compared with control cells (Met-5A/scramble, white histograms). **, p < 0.01; *, p < 0.05, unpaired t test.