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Heparinase treatment of rh-sCD157. Purified rh-sCD157 was treated with heparinase III (Hase III) (2 Sigma units/ml) to remove heparan sulfate (HS) chains potentially decorating the protein. The recombinant protein was run on 10% (w/v) polyacrylamide gel under reducing conditions and stained with Coomassie Brilliant Blue. No shift in molecular weight is appreciable, excluding the presence of heparan sulfate chains. MW, molecular weight markers.
