FIGURE 7.

Antibody blocking and siRNA knockdown of MT1-MMP attenuate CD81-induced melanoma cell motility. A, CD81-transfected MelJuSo cells attached to the Oris cell migration assay plate were pretreated with an anti-MT1-MMP antibody (Ab) or normal mouse IgG for 6 h before removal of the stopper from the Oris plate to initiate cell migration. B and C, CD81 transfectant MelJuSo cells were transfected with siRNAs targeting MT1-MMP. MT1-MMP protein level (B) and motility (C) of siRNA-transfected cells were determined by immunoblotting and Oris cell migration analyses, respectively. Numbers under the immunoblot indicate relative band intensity of MT1-MMP normalized to the actin level. Data shown are the mean ± S.D. from three separate experiments with triplicates per experiment (*, †, and ‡, p < 0.03). Error bars represent S.D. Cont, control.