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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: J Pediatr Gastroenterol Nutr. 2014 Sep;59(3):365–373. doi: 10.1097/MPG.0000000000000441

Figure 4. Effect of −20°C milk storage on IEC death.

Figure 4

Fresh milk was aliquoted and stored at either −80°C (0 weeks) or −20°C for 4, 8, or 12 weeks before being moved to −80°C until testing (total storage time = 20.7 ± 8.3 weeks, max 37.6). After thawing, milk was digested with PBS (white) or lipase (black) (0.1 mg/ml final). N=17. * p<0.0007 vs. Control; § p<0.02 vs. 0 weeks.