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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: J Pediatr Gastroenterol Nutr. 2014 Sep;59(3):365–373. doi: 10.1097/MPG.0000000000000441

Figure 6. Effect of protease digestion and defatting on FFA binding capacity.

Figure 6

Milk from −80 °C storage (20.9 ± 11.3 weeks; min 10.1, max 41.4) was placed in PBS (solid) or digested with 1 mg/ml proteases (slashed). 0 mM or 10 mM oleic acid (in ethanol, 1.5% final) was then added after (white or before (gray) defatting, prior to 60 minute incubation with neutrophils. N=9 milk donors; p<0.017 considered significant. * p<0.01 versus without protease; ‡ p<0.007 versus no oleic acid; § p<0.0002 versus oleic acid added after spinning.