. Author manuscript; available in PMC: 2015 Sep 1.

Published in final edited form as: J Pediatr Gastroenterol Nutr. 2014 Sep;59(3):365–373. doi: 10.1097/MPG.0000000000000441

Table 1.

Percent neutrophil death ^a after 1 h exposure ^b

	with PBS	with Protease	with Lipase	with Lipase + Protease
Fresh Milk ^c	3 ± 2 (0 of 12)	2 ± 1 (0 of 12)^§	6 ± 7 (1 of 12)	7 ± 12 (1 of 12)
Stored 3 days (4°C)	5 ± 4 (0 of 12)	3 ± 2 (0 of 12)	43 ± 23 (10 of 12)^*^‡	28 ± 10 (10 of 12)^*^§^‡
Stored 7 days (4°C)	47 ± 38 (7 of 12)^‡	17 ± 17 (5 of 12)^§^‡	50 ± 19 (12 of 12)^‡	39 ± 19 (10 of 12)^§^‡
Stored 3 days (−20°C)	3 ± 1 (0 of 12)	2 ± 1 (0 of 12)^§^{^}	18 ± 12 (7 of 12)^*^‡^{^}	13 ± 11 (3 of 12)^*^{^}
Stored 7 days (−20°C)	4 ± 2 (0 of 12)^{^}	2 ± 1 (0 of 12)^§^{^}	27 ± 15 (10 of 12)^*^‡^{^}	22 ± 19 (6 of 12)^*^‡^{^}

upper number: Mean % ± SD; lower number in parentheses: Fraction of samples that were cytotoxic - i.e. with death greater than 17%

Samples skimmed after digestion (2 h, 37 °C). Enzyme concentrations: 1 mg/ml. N=12 breast milk donors

Fresh milk data from reference (Penn et al., 2012)

lipase digestion increased death (p<0.003)

^§

protease digestion decreased death (p<0.01)

^‡

increased death vs. fresh milk (p<0.01)

^{^}

less death when stored at −20 °C vs. 4 °C (p<0.01)

p<0.0125 considered significant