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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Clin Exp Metastasis. 2014 May 15;31(6):625–638. doi: 10.1007/s10585-014-9654-5

Figure 1.

Figure 1

Experimental Timetables. (A) Prevention Study: Nude mice were placed on either a low fat (LF) diet, a corn oil (CO) diet, or a stearate (ST) diet 3 weeks prior to injection of cancer cells and remained on separate diets throughout this study. The tumors were allowed to reach an approximate mean tumor diameter of 10–12 mm (253.6–904.8 mm3) at which time the primary tumors were surgically removed (9 weeks postinjection). Chemotherapy with 20 mg/kg paclitaxel, once a week for 3 weeks, was started 1 week after the surgery. The animals were allowed to develop metastases for 3 weeks, sacrificed and the lungs were collected. (B) Treatment Study: All mice were kept on the LF diet until one week after the primary tumors were surgically excised. At this time the corn oil (CO) and stearate (ST) diets were started concomitantly with chemotherapy as described in part A. The resulting dietary groups were: a LF diet group, a CO diet group, a ST diet group, and these same diets plus chemotherapy.