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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Eur J Immunol. 2014 Jun 5;44(8):2331–2339. doi: 10.1002/eji.201344179

Figure 2.

Figure 2

Comparison of the CNTO 95LF antibody mediated cytotoxicity with that induced by parental CNTO 95 antibody. Mutated CNTO 95Ala-Ala antibody was utilized as a negative control. A375 cell line (A) and SKBR3 (B) were used as a target. The 51Cr-labeled target cells were incubated with CD16.NK-92 cells at E:T ratio 5:1 in the presence of indicated concentrations of the antibodies. The specific lysis of the target cells was determined based on the amount of released 51Cr to the culture supernatant. CNTO 95LF, black circles; CNTO 95, open circles; CNTO 95Ala-Ala, open diamonds. Data shown are representative from three to five independent experiments with each condition tested in triplicates in each experiment. Error bars show mean ± SD; *p<0.05, **p<0.001, ***p<0.0001 by two-tailed Student t-test.