Figure 6.
Inhibition of ADCC against tumor cells mediated by CD16.NK-92 cells in the presence of blocking antibodies against β2-integrins. SKBR3 cells were treated with IFN-γ for 48 hours (A) or left untreated (B). The 51Cr-labeled tumor cells were incubated with CD16.NK-92 at E:T ratio 5:1 along with CNTO 95LF antibodies at various concentrations in the presence (black squares) or absence (gray squares) of TS1/18 antibodies (10μg/ml). The percent specific lysis was measured after 4h at 37°C. Results are representative of two independent experiments with each condition tested in triplicates in every experiment and are expressed as mean ± SD, *p<0.05, **p<0.001, ***p<0.0001 by two-tailed Student t-test.