Skip to main content
. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Traffic. 2014 Jul 18;15(9):961–982. doi: 10.1111/tra.12185

Figure 3.

Figure 3

Mpl and Jak2 association is not limited to the plasma membrane. Proximity ligation assay (Duolink) in HEL, K562 and K562Mpl-mKO2 cells. A) K562 cells labelled live for 30 minutes with an anti-CD235a-Atto655 (glycophorin A) antibody before treatment with the Duolink reagents. B) K562Mpl-mKO2 cells non-treated with the Duolink reagents. C-D) HEL cells primed live for 30 minutes with an anti-CD235a-Atto655 antibody before treatment with the Duolink reagents. E-F) K562Mpl-mKO2 cells submitted to the Duolink reaction. White arrow heads are pointing at example of Duolink positive reactions at the plasma membrane, while green arrow heads are pointing at positive Duolink reactions within the cytoplasm.Nuclei are labeled with Hoechst and scale bars = 5 μm. R: Pearson’s correlation coefficient.