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. Author manuscript; available in PMC: 2015 Sep 1.

Published in final edited form as: J Neurochem. 2014 Apr 19;130(5):642–656. doi: 10.1111/jnc.12724

Fig. 6 — PKG mediates adaptation of NMDA-evoked responses following Tat-induced potentiation. A, representative images show neuronal expression of DN-PKG-DsRed2 in a field of neurons treated with Tat for 16 h or 24 h and loaded with fura-2 (scale bar = 50 μM). B, pseudocolor images show peak [Ca²⁺]_i response to 10 μM NMDA (30 s) following 16 h or 24 h Tat treatment. Images are scaled to bar in C and E. C and E, representative traces show NMDA-evoked [Ca²⁺]_i increases for control and Tat-treated cells following 16 h (C) or 24 h (E) exposure to Tat. Recordings from non-expressing cells were from the same imaging field as cells expressing DN-PKG. D and F, Bar graphs show net [Ca²⁺]_i increase evoked by 10 μM NMDA (30 s) in control neurons or neurons treated with 50 ng/mL Tat for 16 h (D) or 24 h (F). *p<0.01; ***p<0.001 relative to respective control; ####p<0.0001 relative to non-expressing, Tat-treated cells as determined by separate, one-way ANOVA with 4 levels for each treatment time followed by Tukey's post test for multiple comparisons.

Inline graphic — PKG mediates adaptation of NMDA-evoked responses following Tat-induced potentiation. A, representative images show neuronal expression of DN-PKG-DsRed2 in a field of neurons treated with Tat for 16 h or 24 h and loaded with fura-2 (scale bar = 50 μM). B, pseudocolor images show peak [Ca²⁺]_i response to 10 μM NMDA (30 s) following 16 h or 24 h Tat treatment. Images are scaled to bar in C and E. C and E, representative traces show NMDA-evoked [Ca²⁺]_i increases for control and Tat-treated cells following 16 h (C) or 24 h (E) exposure to Tat. Recordings from non-expressing cells were from the same imaging field as cells expressing DN-PKG. D and F, Bar graphs show net [Ca²⁺]_i increase evoked by 10 μM NMDA (30 s) in control neurons or neurons treated with 50 ng/mL Tat for 16 h (D) or 24 h (F). *p<0.01; ***p<0.001 relative to respective control; ####p<0.0001 relative to non-expressing, Tat-treated cells as determined by separate, one-way ANOVA with 4 levels for each treatment time followed by Tukey's post test for multiple comparisons.