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. Author manuscript; available in PMC: 2015 May 7.
Published in final edited form as: Neuron. 2014 May 7;82(3):659–669. doi: 10.1016/j.neuron.2014.03.011

Figure 8. SNX27-insensitive GIRK2a expression in DA neurons of SNX27DA KO mice restores normal response to cocaine.

Figure 8

(A) Schematic shows stereotaxic injection of AAV DIO GIRK2a-eYFP or AAV DIO eYFP into VTA of SNX27DA KO mice. (B) Whole-cell recordings from VTA DA neurons show rescue of baclofen-activated GIRK currents (IBaclofen; 300 µM) in SNX27DA KO mice injected with AAV DIO-GIRK2a-eYFP. Scale bar is 100 s and 100 pA. (C) Locomotor activity plots show change in activity following a single cocaine injection (20 mg/kg) for a SNX27DA KO mouse expressing eYFP (left) or GIRK2a-eYFP (right). Starting and ending points indicated by blue and red circles, respectively. Note reduced activity in mouse expressing GIRK2a. (D) Bar graph shows fold-increase in locomotor activity with cocaine (20 mg/kg) for uninfected WT mice (n=5) and SNX27DA KO mice injected with either AAV DIO eYFP (n=3) or AAV DIO GIRK2a-eYFP (n=3) (**p<0.05 eYFP vs GIRK2a-eYFP, unpaired t-test).