Fig. 4.

Degradation of maltoheptaose and starch by ApAmy59 and ApAmy80. Maltoheptaose (A) and starch (B) (0.2 ml, 10 mg/ml in 10 mM acetate, pH 6.0, containing 10 mM CaCl₂) were incubated with 0.18 U of ApAmy59 and ApAmy80 at 37 °C for the time indicated. Fungal amylase from Aspergillus oryzae was incubated in the absence of CaCl₂. The reaction was terminated by heat treatment (95 °C for 2 min) and analyzed by TLC. The intensity of reaction products was analyzed by densitometer.