Table 1.
Properties of the identified enzymes useful in RRBS
| Enzyme | Consensus sequence | Restriction temp. (°C) | Digestion buffer | Heat inactivation (°C) | Methylation sensitive | Catalog number |
|---|---|---|---|---|---|---|
| AluI | AG|CT | 37 | CutSmart | 80 | No | R0137S |
| BfaI | C|TAG | 37 | CutSmart | 80 | No | R0568S |
| HaeIII | GG|CC | 37 | CutSmart | 80 | No | R0108S |
| HpyCH4V | TG|CA | 37 | CutSmart | 65 | No | R0620S |
| MluCI | |AATT | 37 | CutSmart | No | No | R0538S |
| MseI | T|TAA | 37 | CutSmart | 65 | No | R0525S |
| MspI | C|CGG | 37 | CutSmart | No | No | R0106S |
| TaqI | T|CGA | 65 | CutSmart | 80 | dam | R0149S |
| CviQI | G|TAC | 25 | NEB 3.1 | No | No | R0639S |
| CviAII | C|ATG | 25 | CutSmart | 65 | No | R0640S |
| ApeKI | G|CWGC | 75 | NEB 3.1 | No | Yes | R0643S |