Fig. 1.

The impact of IMAC resins on SELEX results. The analysis of enrichment and specificity of aptamer pools selected with the help of Co²⁺-, Cu²⁺-, Ni²⁺-, and Zn²⁺-coated agarose beads (−AB) and Co²⁺-coated polystyrene beads (−PB) against the target. a The enrichment of aptamer pools after I–VIII selection rounds was expressed as the ratio: binding of tested aptamer pool/binding of initial ssDNA library to molecular target. b The specificity of aptamer pools after the eighth SELEX round was expressed as the ratio: binding of aptamer pool to target-containing beads/binding of aptamer pool to empty beads. The presented results are means from three independent experiments. Error bars represent the standard deviation