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Schematic of an inducible strain construction, adk locus and transcription. (A) Construction of S. pneumoniae type 2 adk fucose-inducible strain. (B) adk locus map. Putative promoters of secY, adk, and infA are found at the upstream region. Only putative promoters of adk and infA are shown. (C) mRNA level of the secY and ifnA genes was determined by RT-PCR analyses. Transcription of the adk flanking genes (secY and ifnA genes) was independent from the adk promoter.
