Fig. 8.
(A) Attenuated virulence of the adk mutant in vivo. Groups of mice were infected i.p. or i.n. with approximately 1.5 × 107 CFU of D39 or TTL01 (n = 14–15), and survival time was monitored for 80 h or 14 days for i.p. and i.n. infections, respectively. (B) Attenuated virulence of the adk mutant in vivo in low infection-dose, groups of mice were infected i.p. or i.n. with approximately 1.5 × 104 CFU or 1.5 × 106 CFU of D39 or TTL01 (n = 5), and survival time was monitored for 24 h or 48 h for i.p. and i.n. infections, respectively. (C) Group of mice was infected i.p. with 1.5 × 104 or i.n. with 1.5 × 106 CFU of either D39 or TTL01 for 24 h (i.p.) or 48 h (i.n.), and number of viable bacteria in the blood was determined either 24 h (i.p.) or 48 h (i.n.) post infection after sacrifice, if they were alive, or post-mortem (n = 3–5).