Fig. 2.

No alteration in intracellular GSH content, SOD activity and GSTA4 expression in 3T3-L1 adipocytes was found as the result of FABP4 knockdown. (A and B) Intracellular GSH content and total SOD activity. At 48 h after siRNA transfection, cell lysates were prepared as described in Section 4, and GSH contents (a) and total SOD activity (b) were measured. The intracellular GSH contents and SOD activity per mg protein were calculated. Data represents mean ± SD (n = 3). (C) Western blotting of GSTA4. The protein expression of FABP4, GSTA4 and glyceraldehyde-3-dehydrogenase (GAPDH) was assessed. The chemiluminescent signals were detected using an Image Analyzer (LAS-4000mini).