Fig. 3.

Antioxidant activity of the recombinant FABP4 protein. (A) Measurement of superoxide anion. 1.5 μM of recombinant FABP4 protein and His6 peptide were subjected to quantification of the scavenger activity against superoxide anion. The percentages of the residual superoxide levels against the negative control are shown (n = 3). (B) Quantification of H₂O₂. 5 or 15 μM of recombinant FABP4 control peptide were incubated with H₂O₂, and then, the residual H₂O₂ level was measured. The relative H₂O₂ level in each sample against the negative control (buffer alone) was calculated. Data represent mean ± SD (n = 3). ^†P < 0.005, ^#P < 0.0001 (one-way ANOVA followed by Turkey-Kramer’s HSD test).