Table 1.

Relative level of h2-calponin expression in PC3, PC3-M and transfected PC3-M cells.

Cell line	Level of h2-calponin (% of PC3)
PC3	100.00 ± 6.02
PC3-M	66.07 ± 5.91∗
Transfected PC3-M (low overexpression)a	99.93 ± 6.09†,§
Transfected PC3-M (high overexpression)b	190.46 ± 38.81¶,#,‡

The relative levels of total h2-calponin expressed in PC3, PC3-M and mouse h2-calponin cDNA stable transfected PC3-M cells were quantified from Western blots by normalization to the level of actin shown in parallel SDS-gel. Densitometry comparisons showed that the lower level of h2-calponin in PC3-M versus PC3 cells (^∗P < 0.001) was effectively boosted up by transfective overexpression (^†Low overexpression versus PC3-M: P < 0.001; ^¶High overexpression versus PC3-M: P < 0.001; ^#High overexpression versus PC3: P < 0.001; ^‡High versus low overexpression: P < 0.001. The low overexpression boosted the h2-calponin level in PC3-M cells up to levels similar to that in PC3 cells (^§P = 0.493). The cell proliferation and round up experiments were carried out using transfective low overexpression PC3-M cells for comparison with non-transfected PC3-M and PC3 cells. a, 4 stable cell lines; b, 6 stable cell lines. n = 3 experiments.