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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Eur J Immunol. 2014 Mar 20;44(5):1313–1319. doi: 10.1002/eji.201344079

Figure 3.

Figure 3

miRNA deficiency in TECs causes a breakdown in central tolerance. (A) Mice were immunized with P2 peptide and then harvested 10 days later by flow cytometry following a tetramer pulldown assay. Plots are pregated on DAPI−, NK1.1−, CD11b−, CD11c−, F4/80−, B220−, CD3+ events. Absolute numbers of P2-specific cells are inset within the flow cytometry plots. Tetramer data are pooled from four to five samples in three independent experiments. IRBP−/− mice were included as a positive control for immunization and tetramer pulldown. (B) The IRBP-specific immune response was assessed by an IRBP autoantibody assay in mice immunized with P2 peptide and harvested 21 days later. (C) Eyes harvested from mice in (B) were H&E stained and scored for infiltrates. Scale bars = 200 μm. Data in (B) and (C) is shown as mean ± SEM of 7–8 samples pooled from two independent experiments. * denotes p ≤ 0.05, and ** denotes p ≤ 0.01, Mann–Whitney test.