Figure 6. The effect of sequential administration of tamoxifen in increasing the number of patches derived from Bmi1^High-positive cells.

(a) (left) The testis of Bmi1^creER/+/Rosa26^Rbw/+ mice injected with single dose of tamoxifen. (right) The testis of Bmi1^creER/+/Rosa26^Rbw/+ mice administered 3 sequential injection of tamoxifen. The time course of tamoxifen injection and analyses were shown in the lower panels. Scale bar = 5 mm. (b) The three sequential injections of tamoxifen significantly unregulated the number of patches observed 24 weeks later. P < 0.0005 by unpaired Student's t-test. Red arrows; tamoxifen administration. Black arrows; time points of analyses of the testes. d; days. w; weeks.