Figure 2. OmpU expression of OmpU and ToxR mutants and their sensitivity to ICP2.
Outer membrane fractions were prepared from samples matched by equivalent OD600 units. Samples were separated by SDS-PAGE and subjected to Western blot analysis using rabbit polyclonal antisera against OmpU. The sensitivity of each strain to ICP2_2013_A_Haiti is represented as a histogram of the efficiency of plaquing, which is the plaque count ratio of a mutant V. cholerae strain to that of the wild-type strain. The limit of detection for plaque assays was 10−7.