FIG. 8.

Density of efferent innervation in the IHC area in control versus OC lesion groups. A Innervation density is extracted from confocal z-stacks like that in Fig. 1A, as further described in methods. Control data are from six ears from six animals. OC lesion data are for five ears from five animals. For each group, data are extracted from two adjacent z-stacks from each frequency region in each ear. Mean values (±SEM) are shown. B, C Illustrative confocal images from the 11.3-kHz region of a control (B) and an OC lesion (C) ear. Each image is the maximum projection of a z-stack, and each is oriented as shown in Fig. 1C, D. Arrow in C shows two of the scarce VAT-positive puncta remaining in this z-stack.