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. 2011 Aug 29;134(9):2703–2721. doi: 10.1093/brain/awr200

Figure 3.

Figure 3

Luciferase-3′-untranslated region and overexpression assays verify the interaction of select micro-RNAs with AKR1C1 and AKR1C2 transcripts. Co-transfection of HEK293T cells with vectors encoding firefly luciferase, ligated to the 3′-untranslated region of AKR1C1 (A) or AKR1C2 (B), together with vectors expressing the immature forms of hsa-miR-338, -491 or -155, individually showed significant suppression of luciferase activity, compared with cells transfected with a vector expressing a scrambled micro-RNA sequence. Transfection of Huh7 cells with vectors expressing immature micro-RNA sequences showed significant suppression of AKR1C1 transcripts after miR-338 and miR-491 transfection, compared with cells transfected with a vector expressing a scrambled micro-RNA sequence (C). Moreover, AKR1C2 transcript levels were significantly suppressed in cells overexpressing miR-338, -491, or -155 immature sequences (D), compared with scrambled-miR-expressing cells. Firefly luciferase levels were normalized against Renilla luciferase, which was expressed as an internal control in the target vectors (*P < 0.05, **P < 0.01, Tukey–Kramer Multiple Comparisons Test).