Table 2.
miRNA fold change in multiple sclerosis versus non-multiple sclerosis white matters
| miRNA ID | MS/non-MS ratio | P-value |
|---|---|---|
| Downregulated miRNAs (microarray and PCR) | ||
| hsa-mir-7 | 0.243 | 0.009 |
| hsa-mir-299-5p | 0.349 | 0.018 |
| hsa-miR-135a | 0.371 | 0.017 |
| hsa-mir-218 | 0.396 | 0.006 |
| hsa-miR-129-3p | 0.518 | 0.012 |
| hsa-miR-9 | 0.533 | 0.002 |
| hsa-mir-128 | 0.534 | 0.019 |
| hsa-miR-130A | 0.549 | 0.023 |
| hsa-mir-126 | 0.603 | 0.039 |
| hsa-miR-335 | 0.616 | 0.037 |
| hsa-miR-98 | 0.663 | 0.030 |
| miRNA ID | Fold change (MS/control) | P-value |
|---|---|---|
| Upregulated miRNAs (microarray and PCR) | ||
| hsa-miR-25 | 1.677 | 0.026 |
| hsa-miR-505* | 1.934 | 0.000 |
| hsa-miR-320b | 1.986 | 0.003 |
| hsa-miR-320a | 1.995 | 0.003 |
| hsa-miR-338-3p | 2.119 | 0.036 |
| hsa-miR-181b | 2.182 | 0.013 |
| hsa-mir-125b1 | 2.218 | 0.018 |
| hsa-miR-92a | 2.292 | 0.003 |
| hsa-mir-155 | 2.451 | 0.024 |
| hsa-miR-584 | 2.456 | 0.013 |
| hsa-miR-219-2-3p | 2.749 | 0.008 |
| hsa-miR-338-5p | 2.949 | 0.006 |
| hsa-miR-219-5p | 2.989 | 0.028 |
| hsa-miR-142-5p | 3.461 | 0.024 |
As shown in Fig. 1A, micro-RNAs (miRNAs) showing >1.5-fold change at microarray analysis were further analysed with real-time reverse transcription–PCR. The upper and lower panels show miRNAs with altered expression confirmed by PCR analysis, showing also a minimum 1.5-fold change at the PCR level. MS = multiple sclerosis.