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. Author manuscript; available in PMC: 2015 Aug 14.
Published in final edited form as: Cell. 2014 Aug 14;158(4):861–873. doi: 10.1016/j.cell.2014.06.028

Figure 5. Genome-wide correlation between replication and Rec114 accumulation.

Figure 5

(A) Delaying replication delays Rec114 accumulation on the entire left arm of chr III. Rec114 ChIP and input DNA were sequenced and read coverage (ChIP/input) was scaled with qPCR data (Figure S5A). Chr VI is an internal control. Dashed lines are centromeres.

(B) Reproducibility between ARS+ and arsΔ cultures for timing of Rec114 ChIP (left) and replication (right). Left: each point is the tRec114 for a Rec114 ChIP peak (Figure S5E). Right: replication indices (from ChIP input DNA) in non-overlapping 5-kb windows.

(C) Correlation between replication indices (non-overlapping 5-kb windows) from total genomic DNA or ChIP inputs.

(D) Correlation between timing of replication and Rec114 ChIP. Points are Rec114 peaks.

(E) Spatial patterns for timing of replication and Rec114 ChIP on representative chromosomes. Points are Rec114 peaks. Dashed gray lines, centromeres; dashed blue lines, qPCR primer pairs.