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A mixture of O-linked glycans from a 1:1 mixture of light/heavy labeled mESCs were mixed together and analyzed on an LTQ-Orbitrap. A. Quantification of the monosialyated T-antigen in a 8 m/z window (2 nitrogens). B. Quantification of a disialyated core 2 structure in a 9 m/z window (4 nitrogens).
