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. Author manuscript; available in PMC: 2014 Aug 22.
Published in final edited form as: Biochemistry. 2012 Sep 5;51(37):7250–7262. doi: 10.1021/bi300653m

Figure 3. Soluble protein expression comparison of EB sequences with commonly used fusions.

Figure 3

A bar graph showing the soluble expression performance of EB60A, EB60B, EB144 and EB250 (dark bars) with Trx, GST, NusA and MBP fusions (light bars) marketed to enhance soluble protein expression. B. Western blot analysis of the protein TIMP2 hybridized to the various fusion partners (anti-6x His blot, G-18 from Santa Cruz Biotechnologies).