. Author manuscript; available in PMC: 2014 Aug 22.

Published in final edited form as: Biochemistry. 2012 Sep 5;51(37):7250–7262. doi: 10.1021/bi300653m

Table 3.

Compilation of disorder and solubility predictions for various protein fusions

Protein	Percent^a Disordered		Mean^b	WH^c	ProSolI^d	SolPro^e
			Hydropathy	Solubility	Solubility	Solubility
	VLXT	VSL2		Predictor	Predictor	Predictor
EB60A	100%	100%	0.2281	97% soluble	0.88 soluble	0.97 soluble

EB60B	100%	100%	0.2133	97% soluble	0.89 soluble	1.00 soluble

EB144	100%	100%	0.2640	86% insoluble	0.88 soluble	0.97 soluble

EB250	100%	100%	0.3424	92% insoluble	0.87 soluble	0.97 soluble

GST	12%	14%	0.4572	58% soluble	0.58 insoluble	0.78 insoluble

MBP	11%	17%	0.4640	52% insoluble	0.77 soluble	0.94 soluble

NusA	47%	20%	0.4423	95% soluble	0.66 soluble	0.58 soluble

Trx	6.4%	12%	0.028	72.6% soluble	0.36 insoluble	0.89 soluble

^a.

Percentage of amino acids found disordered using the PONDR^® predictors VLXT (35, 36), and VSL2 (37, 38).

^b.

A measure that distinguishes ordered from disordered proteins (40).

^c.

The revised Wilkinson-Harrison solubility predictor (8).

^d.

The soluble probability value ranges from values 0–0.6 being insoluble to values ≥0.6–1.0 being soluble (26).

^e.

The probability value for the soluble/insoluble designation range from 0.5–1.0 (25).