Table 4.
Summary of Solubility Data for twenty test protein candidates
| Protein | 6xHis | MBP | EB60A | EB60B | EB144 | EB250 |
|---|---|---|---|---|---|---|
| 408 | 55 ± 2 % | 66 ± 6 % | 68 ± 5 % | 79 ± 7 % | 71 ± 15 % | 89 ± 5 % |
| 2141 | 18 ± 9 % | 17 ± 18 % | 39 ± 2 % | 33 ± 7 % | 56 ± 17 % | 59 ± 9 % |
| CATA9 | 3 ± 2 % | 0 ± 0 % | 1 ± 1 % | 0 ± 1 % | 58 ± 6 % | 65 ± 5 % |
| EFNA1 | 4 ± 3 % | 13 ± 4 % | 13 ± 4 % | 4 ± 1 % | 51 ± 7 % | 54 ± 7 % |
| FLCN | 2 ± 3 % | 0 ± 0 % | 0 ± 0 % | 7 ± 8 % | 94 ± 7 % | 88 ± 3 % |
| GADD45 | 45 ± 8 % | 55 ± 2 % | 57 ± 7 % | 62 ± 6 % | 92 ± 4 % | 94 ± 3 % |
| GFP | 56 ± 12 % | 35 ± 1 % | 52 ± 8 % | 45 ± 2 % | 84 ± 14 % | 87 ± 15 % |
| ID2 | 42 ± 6 % | 91 ± 8 % | 78 ± 2 % | 90 ± 11 % | 97 ± 4 % | 83 ± 4 % |
| IL-7 | 0 ± 0 % | 10 ± 1 % | 22 ± 2 % | 16 ± 2 % | 25 ± 6 % | 43 ± 2 % |
| IL-13 | 18 ± 1 % | 48 ± 3 % | 81 ± 10 % | 97 ± 3 % | 84 ± 7 % | 84 ± 11 % |
| IL-21 | 0 ± 0 % | 30 ± 5 % | 46 ± 6 % | 57 ± 6 % | 70 ± 8 % | 79 ± 9 % |
| MAD | 4 ± 2 % | 21 ± 2 % | 41 ± 2 % | 43 ± 3 % | 86 ± 2 % | 92 ± 2 % |
| MTHFS | 17 ± 3 % | 10 ± 2 % | 15 ± 3 % | 31 ± 9 % | 75 ± 7 % | 36 ± 9 % |
| MSTN | 0 ± 0 % | 25 ± 3 % | 27 ± 1 % | 45 ± 2 % | 55 ± 16 % | 74 ± 2 % |
| PHB | 0 ± 0 % | 27 ± 11 % | 26 ± 8 % | 6 ± 2 % | 39 ± 5 % | 43 ± 4 % |
| SNW1 | 7 ± 4 % | 4 ± 5 % | 38 ± 2 % | 58 ± 10 % | 82 ± 3 % | 85 ± 2 % |
| TEV | 2 ± 3 % | 66 ± 3 % | 79 ± 2 % | 89 ± 11 % | 89 ± 1 % | 57 ± 27 % |
| TIMP2 | 2 ± 4 % | 30 ± 4 % | 45 ± 2 % | 37 ± 4 % | 41 ± 3 % | 66 ± 3 % |
| TNSF13b | 2 ± 3.5 % | 1 ± 1.2 % | 26 ± 4 % | 30 ± 5 % | 30 ± 5 % | 31 ± 2 % |
| WAG2 | 0 ± 0 % | 9 ± 3 % | 6 ± 4 % | 7 ± 6 % | 46 ± 1 % | 89 ± 8 % |
| % p-value vs. His <0.05 |
na | 65% | 75% | 75% | 95% | 100% |
| % p-value vs. MBP <0.05 |
20% | na | 50% | 50% | 85% | 85% |