Adult bHR and bLR offspring that were maternally separated (MS) or remained undisturbed (CON) in early life were tested in the Open Field (OF), the Elevated Plus Maze (EPM), and the Light Dark Box (LDB). bLRs consistently showed greater anxiety compared to bHRs, spending less time in the center of the OF (A), less time in the open arms of the EPM (C), and less time in the light compartment in the LDB (E). bLRs also showed greater latency to initially enter these anxiogenic regions compared to bHRs (B, D, F). A history of MS had no impact on any of these measures. Groups were compared using a 2-way ANOVA and Fisher’s post hoc comparisons where necessary; * indicates p<0.05; ***indicates p<0.0001