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. Author manuscript; available in PMC: 2014 Aug 22.
Published in final edited form as: Cancer Res. 2012 Sep 10;72(21):5625–5634. doi: 10.1158/0008-5472.CAN-12-1305

Figure 5.

Figure 5

MED1 phosphorylation is required for the recruitment of NCoR and SMRT to TFF1 promoter by tamoxifen. A, schematic diagram of wild-type (WT) MED1 and T1032A/ T1457A double-mutated (DM) MED1. B, Western blot analyses of MED1 protein levels after infections with lentivirus expressing WT or DM MED1 into MCF-7/HER2 cells pretreated with control scramble or MED1 shRNA. C, cells described in B were further treated with 1 μmol/L TAM or vehicle for 45 minutes and then subjected to ChIP assays using IgG (negative control) or antibodies for MED1, p-MED1, NCoR, or SMRT. (* , P < 0.05).