Skip to main content
. Author manuscript; available in PMC: 2014 Aug 22.
Published in final edited form as: Cancer Res. 2012 Sep 10;72(21):5625–5634. doi: 10.1158/0008-5472.CAN-12-1305

Figure 6.

Figure 6

Knockdown of MED1 also inhibits the expression of EGF-ERα target genes. A, diagram for the regulation of ERα-dependent E2-ERα and EGF-ERα target genes by E2 and EGF. B, MCF-7 and MCF-7/ HER2 cells were treated with 50 μmol/L AG825 or vehicle for 1 hour and subject to chromatin IP experiments using control IgG or anti-MED1 antibodies. C, total RNA was extracted from MCF-7/ HER2 cells treated with control scramble or MED1 shRNA, and the expression of EGF-ERα target genes ACP6 and LIF were measured by real-time RT-PCR and normalization to that of GAPDH. (*, P < 0.05).