Table 1. PCR primers.

Gene	Prime sequences (Forward and Reverse)	Tm (°C)*
Collagen Type 1 (α1)	5′-CCGGAAACAGACAAGCAACCCAAA-3′ 3′-AAAGGAGCAGAAAGGGCAGCATTG-5′	73.2 71.8
Osteonectin	5′-TTCTGCCTGGAGACAAGGTGCTAA-3′ 3′-TCTGTTACTTCCCTTTGCCCACCT-5′	69.8 69.4
PPARγ2	5′-CTGTTTGCCAAGCTGCTCCAGAAA-3′ 3′-AAGAAGGGAAATGTTGGCAGTGGC-5′	72.1 71.6
Nkx2.5	5′-GGTGGAGCTGGAGAAGACAGA-3′ 3′-CGACGCCGAAGTTCACGAAGT-5′	65.7 67.8
GATA4	5′-AGCAGCTCCTTCAGGCAGT-3′ 3′-GCCCATGGCCAGACATC-5′	66.2 62.9
MEF2C	5′-GATCATCTTCAACAGCACC-3′ 3′-GTTCAATGCCTCCACGA-5′	59.5 60.5
CACNA1c	5′-CAGAAACTACAGGAGAAGAGG-3′ 3′-AAGAAGAGGATCAGGTTGGT-5′	59.5 60.5
(β-Actin	5′-AAGATCAAGATCATTGCTCCTC-3′ 3′-GGACTCATCGTACTCCTG-5′	61.2 59.5

^*Tms were calculated by NIH PerlPrimer software.