Table 1. PCR primers.
Gene | Prime sequences (Forward and Reverse) | Tm (°C)* |
---|---|---|
Collagen Type 1 (α1) | 5′-CCGGAAACAGACAAGCAACCCAAA-3′ 3′-AAAGGAGCAGAAAGGGCAGCATTG-5′ |
73.2 71.8 |
Osteonectin | 5′-TTCTGCCTGGAGACAAGGTGCTAA-3′ 3′-TCTGTTACTTCCCTTTGCCCACCT-5′ |
69.8 69.4 |
PPARγ2 | 5′-CTGTTTGCCAAGCTGCTCCAGAAA-3′ 3′-AAGAAGGGAAATGTTGGCAGTGGC-5′ |
72.1 71.6 |
Nkx2.5 | 5′-GGTGGAGCTGGAGAAGACAGA-3′ 3′-CGACGCCGAAGTTCACGAAGT-5′ |
65.7 67.8 |
GATA4 | 5′-AGCAGCTCCTTCAGGCAGT-3′ 3′-GCCCATGGCCAGACATC-5′ |
66.2 62.9 |
MEF2C | 5′-GATCATCTTCAACAGCACC-3′ 3′-GTTCAATGCCTCCACGA-5′ |
59.5 60.5 |
CACNA1c | 5′-CAGAAACTACAGGAGAAGAGG-3′ 3′-AAGAAGAGGATCAGGTTGGT-5′ |
59.5 60.5 |
(β-Actin | 5′-AAGATCAAGATCATTGCTCCTC-3′ 3′-GGACTCATCGTACTCCTG-5′ |
61.2 59.5 |
Tms were calculated by NIH PerlPrimer software.