Top, Transaxial cryosection, digital autoradiograph (ARG), and digital autoradiograph superimposed on cryosection (overlay) of a representative rat. Bottom, Schema of the cryosection and transaxial [18F]-FHBG microPET image superimposed on [13N]-ammonia image at the corresponding slice level of the same rat. Two radioactive foci are clearly identified on the autoradiograph, which also can be recognized on the microPET image.