Figure 1.

Typical shrink-and-swell response of a mature rhesus monkey oocyte during exposure to a penetrating cryoprotectant additive. A: Oocyte held in isotonic medium within the tip of an outer pipette, just prior to cryoprotectant exposure. Subsequent images show the same oocyte after exposure to a 1.1 M solution of ethylene glycol for 5 sec (B), 1 min (C), and 16 min (D). Scale bar represents 20 µm. [See color version online at www.interscience.wiley.com.]