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. Author manuscript; available in PMC: 2014 Aug 22.
Published in final edited form as: Adv Virus Res. 2013;86:1–36. doi: 10.1016/B978-0-12-394315-6.00001-5

Fig. 5.

Fig. 5

20S and 23S RNA viral genomes. A. Total RNA extracted from induced cells containing no viruses (lane 1), 20S RNA alone (lane 2), or 20S and 23S RNAs (lane 3). Ethidium bromide staining of an agarose gel is shown. 18S and 25S rRNAs are indicated. B. 20S and 23S RNA genomes and the encoded proteins, p91 and p104. P91 and p104 contain the consensus sequences (A-D) for RNA-dependent RNA polymerases, which are most closely related to those of RNA coliphages. 1-3 indicates amino acid stretches conserved between p91 and p104. C. Diagram of a launching plasmid. The complete sequence of the 20S or 23S RNA genome is inserted downstream of the constitutive PGK1 promoter (PGK1). The hepatitis delta virus ribozyme (R) is fused directly to the 3’ end of the viral genome. Thin lines indicate sequences derived from the vector.