Extended Data Figure 5. Validation of metabolomics analyses.

a. The reproducibility of the untargeted metabolomics platform was validated from two separate runs of 6 serum samples. The Spearman’s rank correlations are between 0.9 and 0.94. The duplicate pair with the lowest correlation (Spearman’s r=0.90) is shown. b. The raw intensity of samples was subject to normalization with median centering and inter-quartile range (IQR) scaling. The resulting data show equal distribution among different groups of samples. White bar represents samples obtained in the light cycle and black bar for those in the dark cycle.