Skip to main content
. 2014 Aug 22;9(8):e105434. doi: 10.1371/journal.pone.0105434

Table 2. Classification of candidate Ag's.

Condition Abbreviation Concentration (µg/ml) Relevance for MS
No antigen No Ag Negative control; background proliferation and cytokine secretion
Brain homogenate BH 5.0 Auto-Ag; derived from snap-frozen MS brain <8 hrs post-mortem
Human myelin Myelin 2.5 Auto-Ag; derived from snap-frozen MS brain <8 hrs post-mortem
Differentiated MO3.13 cell lysate MO313 1.0 Auto-Ag; human oligodendroglial cell line post-exposure to H2O2
SK-N-SH cell lysate SKNSH 5.0 Auto-Ag; human neuronal cell line post-exposure to UVA light
EBV viral lysate EBV 1.0 Foreign Ag; epidemiologically linked to MS
HHV-6 viral lysate HHV6 1.0 Foreign Ag; linked to MS by previous observational studies
CMV viral lysate CMV 1.0 Foreign control Ag
EBNA-1458-641 peptide EBNA 1.0 Immunodominant EBV protein previously linked to MS
CSF EBV-B cell lysate CSF Bc 5.0 Combination of EBV and auto-Ag; to test anti-idiotypic interactions
Peripheral EBV-B cell lysate Periph Bc 5.0 Combination of EBV and auto-Ag; to test anti-idiotypic interactions

Optimal concentrations of candidate Ag's were selected based on pilot experiments.