Table 2. Classification of candidate Ag's.
Condition | Abbreviation | Concentration (µg/ml) | Relevance for MS |
No antigen | No Ag | Negative control; background proliferation and cytokine secretion | |
Brain homogenate | BH | 5.0 | Auto-Ag; derived from snap-frozen MS brain <8 hrs post-mortem |
Human myelin | Myelin | 2.5 | Auto-Ag; derived from snap-frozen MS brain <8 hrs post-mortem |
Differentiated MO3.13 cell lysate | MO313 | 1.0 | Auto-Ag; human oligodendroglial cell line post-exposure to H2O2 |
SK-N-SH cell lysate | SKNSH | 5.0 | Auto-Ag; human neuronal cell line post-exposure to UVA light |
EBV viral lysate | EBV | 1.0 | Foreign Ag; epidemiologically linked to MS |
HHV-6 viral lysate | HHV6 | 1.0 | Foreign Ag; linked to MS by previous observational studies |
CMV viral lysate | CMV | 1.0 | Foreign control Ag |
EBNA-1458-641 peptide | EBNA | 1.0 | Immunodominant EBV protein previously linked to MS |
CSF EBV-B cell lysate | CSF Bc | 5.0 | Combination of EBV and auto-Ag; to test anti-idiotypic interactions |
Peripheral EBV-B cell lysate | Periph Bc | 5.0 | Combination of EBV and auto-Ag; to test anti-idiotypic interactions |
Optimal concentrations of candidate Ag's were selected based on pilot experiments.