Figure 7. Stress phenotypes of the phs1 mutant of Sporobolomyces suggest that defects in other cellular processes can impair the formation of spores.

10-fold serial dilutions of overnight cultures of Sporobolomyces wild type (IAM 13481), mutant phs1 (GI209), complementing strain phs1 + PHS1 (AIS15) and uracil prototroph phs1+ URA5 (AIS13) were plated onto YPD media without or with stress agents, and cultured 3–4 days. Stress agents were caspofungin (50 µg/ml), congo red (0.4%), sodium dodecyl sulfate (SDS, 0.015%) and sodium chloride (NaCl, 1 M).