Strains
|
|
|
DH5α |
E. coli supE4 ΔlacU169 (φ80 ΔlacZ ΔM15) hsdR17, recA1, endA1, gyrA96, thi-1, relA1
|
Clontech |
MC1061 |
E. coli F−
Δ(ara-leu)7697 [araD139] B/r Δ(codB-lacI)3 galK16 galE15 λ− e14− mcrA0 relA1 rpsL150 (StrR) spoT1 mcrB1 hsdR2(r−m+)
|
CGSC |
BL21 (DE3) pLysS |
E. coli F− dcm ompT lon hsdS(rB−mB−) galλ(DE3) carring pLysS plasmid, CmR
|
Stratagene |
LT2 |
S. Typhimurium wild type strain |
ATCC |
UA1907 |
S. Typhimurium ΔcheWΩcat. CmR
|
This work |
UA1908 |
S. Typhimurium ΔcheW
|
This work |
UA1910 |
S. Typhimurium ΔcheR
|
This work |
UA1915 |
S. Typhimurium ΔcheR ΔcheW
|
This work |
UA1913 |
S. Typhimurium ΔcheR ΔrecA
|
This work |
UA1927 |
S. Typhimurium ΔrecAΩ,cat. CmR
|
This study |
UA1928 |
S. Typhimurium ΔcheB
|
[29]
|
UA1929 |
S. Typhimurium ΔcheY
|
[29]
|
UA1930 |
S. Typhimurium ΔcheW ΔrecA
|
This work |
UA1931 |
S. Typhimurium ΔrecA
|
This work |
Plasmids
|
|
|
pKOBEGA |
Vector containing the λ Red recombinase system, Ampr, temperature sensitive |
Generous gift of Prof. G. M. Ghigo, [33]
|
pKD3 |
Vector carrying FRT-Cm construction, AmpR, CmR
|
[31]
|
pCP20 |
Vector carrying FLP system, OriVts, AmpR
|
[31]
|
pGEM-T |
Cloning Vector; AmpR
|
Promega |
pGEX 4T-1 |
Expression vector carrying the Ptac IPTG - inducible promoter and the lacIq gene; GST fusion tag, AmpR
|
Amersham Biosciences |
pUA1108 |
pGEX 4T-1 derivative plasmid carrying without the GST fusion tag, carrying only the Ptac promoter and the lacIq gene; used as overexpression vector, AmpR
|
This work |
pUA1109 |
pUA1108 derivative containing the native S. Typhimurium recA gene under the control of the Ptac promoter, AmpR. |
This work |
pUA1127 |
pUA1108 derivative vector carrying the eYFP::cheR fusion, AmpR
|
This work |
pUA1130 |
pUA1108 derivative containing the native S. Typhimurium recA gene under the control of the Ptac promoter, AmpR. |
This work |
pUA1131 |
pUA1108 derivative overexpression vector carrying the cheW-FLAG gene |
This work |
pB2HΔα |
pACYCDuet-1 derivative vector with the E. coli Δα β-galactosidase fragment under the control of the Ptac promoter |
BCCM/LMBP, [36]
|
pB2HΔω |
pACYCDuet-1 derivative vector with the E. coli Δω β-galactosidase fragment under the control of the Ptac promoter |
BCCM/LMBP, [36]
|
pUA1114 |
pB2HΔα derivative vector contaning the Δα-recA fusion |
This work |
pUA1115 |
pB2HΔω derivative plasmid contaning the Δω-recA fusion |
This work |
pUA1116 |
pB2HΔα derivative vector contaning the Δα-cheW fusion |
This work |
pUA1117 |
pB2HΔω derivative plasmid contaning the Δω-cheW fusion |
This work |
pUA1118 |
pB2HΔα derivative plasmid contaning the Δα-amyA fusion, used as a control in two hybrid assays. |
This work |
pUA1119 |
pB2HΔω derivative plasmid contaning the Δω-dnaE fusion, used as a control in two hybrid assays. |
This work |