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. 2014 Aug 22;9(8):e105326. doi: 10.1371/journal.pone.0105326

Figure 2. EphB4 provides survival advantage to bladder tumor cells.

Figure 2

A, Tumor cell line expressing EphB4 (5637) was treated with various doses of EphB2 or EphB4 siRNA and viable cell number was determined using MTT assay. Cells were treated for 48 hrs. Data are presented as mean ± standard deviation (n = 3). Knockdown of EphB4 but not EphB2 significantly reduced viable tumor cell number. Asterisk indicates P<0.002, as determined by an unpaired 2-tail student T-test. B, Protein lysates were examined for EphB4 levels. EphB4 expression was effectively knocked down with EphB4 siRNA (β-actin as loading control). C, MTT assay of the normal urothelial cell line PD07I showed no effect on cell viability with EphB2 or EphB4 knockdown with varying siRNA dosages. D, Protein lysates were measured by Western blot analysis, showing almost complete abrogation of EphB2 protein with EphB2 siRNA.