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. 2014 Aug 22;9(8):e105768. doi: 10.1371/journal.pone.0105768

Figure 5. Flow cytometric analysis of reactive oxygen species (ROS) in BA-treated cells.

Figure 5

HeLa cells were treated with 15 µmol/L, 30 µmol/L BA for 48 h and then incubated with 10 mmol/L DCFH-DA for 40 min. The fluorescent intensity of DCFH was measured by flow cytometry. (A) Actual spectra from a representative single experiment. (B) The fluorescence intensity of stained cells was determined by flow cytometry. Columns show mean values of three experiments (±SD). **p<0.01 compared with the control group (0 µmol/L BA).