Table 1. Oligonucleotides and expected sizes of PCR products for qRT-PCR analysis of differentiation marker genes.
Primer sequence (5′ -> 3′) | Product size(bp) | Anneal Temperature (°C) | |
bax | AGAAGGCACTAATCAAGTCAAGGTC | 438 | 61 |
TTTCTGACGGCAACTTCAACTGGGG | |||
bcl-2 | AAGGGCATTTTTCCCATCGCTGTCC | 350 | 61 |
TCTGTGCCTGTAAACATAGATTCGC | |||
14-3-3β | GTTTGTTGTCTCCAGATGCCACTTC | 146 | 61 |
GAAGAAGCAGCAGATGGGCAAAGAG | |||
14-3-3ε | TTCTTTATTCTGCTCTTCACCGTCA | 253 | 61 |
AGAACTTCCACCAACGCATCCTATT | |||
GAPDH | TTGGTTGAGCACAGGGTACTTTATT | 166 | 61 |
CACCAGGTGGTCTCCTCTGACTTCA |