Skip to main content
. 2014 Aug 22;9(8):e105768. doi: 10.1371/journal.pone.0105768

Table 1. Oligonucleotides and expected sizes of PCR products for qRT-PCR analysis of differentiation marker genes.

Primer sequence (5′ -> 3′) Product size(bp) Anneal Temperature (°C)
bax AGAAGGCACTAATCAAGTCAAGGTC 438 61
TTTCTGACGGCAACTTCAACTGGGG
bcl-2 AAGGGCATTTTTCCCATCGCTGTCC 350 61
TCTGTGCCTGTAAACATAGATTCGC
14-3-3β GTTTGTTGTCTCCAGATGCCACTTC 146 61
GAAGAAGCAGCAGATGGGCAAAGAG
14-3-3ε TTCTTTATTCTGCTCTTCACCGTCA 253 61
AGAACTTCCACCAACGCATCCTATT
GAPDH TTGGTTGAGCACAGGGTACTTTATT 166 61
CACCAGGTGGTCTCCTCTGACTTCA