Table 2. Proteins identified for which the level of expression changed significantly (p<0.05) after the treatment of 30 µmol/L BA for 48 h.
Spots NO.a | Protein Nameb | Abbreviate namec | Accession NO.d | MW (kDa)/pIe | Protein Scoref | Regulationg |
16 | phosphomevalonate kinase | PMVK | gi|5729980 | 5.56/22152 | 466 | ↑ |
27 | interferon-gamma inducing factor precursor | IL18 | gi|1899242 | 4.54/22466 | 88 | ↑ |
146 | glyoxalase domain-containing protein 4 | GLOD4 | gi|4929769 | 8.99/55832 | 136 | ↑ |
613 | 78 kDa glucose-regulated protein precursor | HSPA5 | gi|386758 | 5.03/72185 | 1064 | ↑ |
617 | lamin A protein, partial | LMNA | gi|386856 | 6.4/57686 | 151 | ↑ |
248 | cargo selection protein TIP47 | PLIN3 | gi|3095186 | 5.00/47254 | 53 | ↑ |
372 | alpha-enolase isoform 1 | ENO1 | gi|4503571 | 7.01/47481 | 140 | ↓ |
409 | ruvB-like 1 | RUVBL1 | gi|4506753 | 6.02/50538 | 89 | ↓ |
526 | UDP-glucose 6-dehydrogenase isoform 1 | UGDH | gi|4507813 | 6.73/55674 | 279 | ↓ |
673 | elongation factor 2 | EEF2 | gi|4503483 | 6.41/96246 | 722 | ↓ |
868 | heterogeneous nuclear ribonucleoprotein F | HNRNPF | gi|4826760 | 5.38/45985 | 88 | ↓ |
932 | elongation factor 2 isoform X1 | EEF2 | gi|530425157 | 5.77/63494 | 234 | ↓ |
991 | guanine nucleotide-binding protein subunit beta-2-like 1 | GNB2L1 | gi|5174447 | 7.6/35511 | 167 | ↓ |
1068 | TIMM50 protein | TIMM50 | gi|14290586 | 8.56/39014 | 280 | ↓ |
1077 | guanine deaminase isoform b | GDA | gi|4758426 | 5.44/51484 | 345 | ↓ |
1085 | adenylyl cyclase-associated protein 1 | CAP1 | gi|5453595 | 8.07/51926 | 67 | ↓ |
1087 | alpha-tubulin | TUBA1A | gi|37492 | 5.02/50810 | 68 | ↓ |
1097 | motor protein | IMMT | gi|516764 | 5.71/79830 | 252 | ↓ |
1120 | 6-phosphogluconate dehydrogenase, decarboxylating | PGD | gi|40068518 | 6.8/53619 | 217 | ↓ |
81 | heat shock protein 27 | HSPB1 | gi|662841 | 5.56/27458 | 345 | ↓ |
120 | endoplasmic reticulum resident protein 29 isoform 1 precursor | ERP29 | gi|5803013 | 6.35/32445 | 93 | ↓ |
127 | nudix hydrolase NUDT5 | NUDT5 | gi|6694937 | 4.76/33277 | 49 | ↓ |
128 | microtubule-associated protein RP/EB family member 1 | MAPRE1 | gi|6912494 | 5.00/33773 | 407 | ↓ |
361 | alpha-internexin | INA | gi|14249342 | 5.33/57148 | 84 | ↓ |
542 | chain A, Horf6 A novel human peroxidase enzyme | PRDX6 | gi|3318841 | 6.5/27400 | 566 | ↓ |
573 | BTB/POZ domain-containing protein KCTD12 | KCTD12 | gi|19923973 | 5.34/37615 | 52 | ↓ |
626 | heterogeneous nuclear ribonucleoprotein F | HNRNPF | gi|4826760 | 5.36/49502 | 117 | ↓ |
655 | chain A, tapasin ERp57 heterodimer | PDIA3 | gi|220702506 | 5.82/55312 | 336 | ↓ |
821 | phosphoglycerate mutase 1 | PGAM1 | gi|4505753 | 6.59/29254 | 291 | ↓ |
823 | 14-3-3 protein beta | YWHAB | gi|4507949 | 4.67/29378 | 273 | ↓ |
842 | 14-3-3 protein epsilon | YWHAE | gi|5803225 | 4.53/31499 | 322 | ↓ |
885 | elongation factor 1-delta isoform 2 | EEF1D | gi|25453472 | 4.8/38175 | 152 | ↓ |
942 | dual specificity mitogen-activated protein kinase kinase 2 | MAP2K2 | gi|13489054 | 6.15/47554 | 73 | ↓ |
970 | mutant beta-actin (beta'-actin) | ACTB | gi|28336 | 5.16/51529 | 67 | ↓ |
981 | T-complex protein 1, Beta subunit (TCP-1-BETA) | CCT1 | gi|1871210 | 6.4/52518 | 45 | ↓ |
993 | selenium-binding protein 1 isoform 1 | SELENBP1 | gi|16306550 | 4.15/53639 | 73 | ↓ |
1109 | unnamed protein product | P4HB | gi|35655 | 4.75/55600 | 230 | ↓ |
Assigned spot number as indicated in Fig. 2.
Each protein spot was identified based on mass spectra of tryptic peptides obtained by MALDI-TOF mass spectrometry.
Abbreviate name is connected with signal network.
Accession numbers of the identified protein spots were obtained from the NCBInr-human data base.
Theoretical molecular mass (Da) and pI.
Mascot score reported after searching against the NCBInr database.
The protein spots altered over 1.5-folds.