Figure 15. AT₁ and AT₂ receptor binding comparison.

Comparison of ¹²⁵I-SI Ang II binding to the AT₁ and AT₂ receptors of neurolysin KO (right panels) and WT (left panels) mouse strain brains in the presence of PD123319 or losartan, respectively. Approximate coordinates relative to Bregma: +0.98 mm (histology and autoradiograms) for KO, and Bregma +0.92 (histology), +0.86 (AT₁), and +0.98 mm (AT₂ and non-specific) for WT. Top row of panels are thionin-stained coronal sections adjacent to the sections used to generate the autoradiograms for “total” ¹²⁵I-SI Ang II binding to the AT₁ receptor (second row), AT₂ receptor (third row), and “non-specific” ¹²⁵I-SI Ang II binding (fourth row), represented in pseudocolor. The vertical calibration bar represents the relationship between binding density of ¹²⁵I-SI Ang II and the color spectrum. The horizontal calibration bar in the upper left panel  = 2 mm. This pattern is repeated for Figures 16 and 17.