Figure 1. Sensitivity of the real-time polymerase chain reaction assay for detecting Plasmodium vivax in a blood sample (800 parasites/µL) (A) and correlation between C_t value and parasitemia (B).

(A) Plot of the mean cycle threshold (C_t) values against P. vivax DNA amount extracted from blood samples with a parasitemia of 800 parasites/µL using 2-fold dilutions (2⁰–2¹³ dilutions). The plot of C_t values and DNA amount fits a linear function (R ² = 0.9703). (B) Log values of parasitemia (parasites/µL) against C_t values were plotted for 54 blood samples infected with P. vivax.