Table 3. Sensitivity, specificity, and agreement of real-time PCR, LAMP, and multiplex PCR using nested PCR results as the gold standard.
Real-time PCR | LAMP | Multiplex PCR | |
Sensitivitya (%) | 99.6 (97.7–100) | 99.6 (97.7–100) | 99.3 (97.1–99.9) |
Specificitya (%) | 100 (90.2–100) | 100 (90.2–100) | 97.8 (86.8–99.9) |
Agreementa,b (%) | 99.9 (98.0–100) | 99.9 (98.0–100) | 99.1 (97.0–99.8) |
a: 95% confidence intervals are shown in parentheses.
b: calculated by DAG_Stat and VassarStats (http://vassarstats.net/).
LAMP: loop-mediated isothermal amplification; PCR: polymerase chain reaction.